Nuclear exosome regulator NRDE2
nuclear speck
Human nuclear RNAi-defective 2 (NRDE2) is an essential RNA splicing factor.
The accurate inheritance of genetic material is a basic necessity in all domains of life and an unexpectedly large number of RNA processing factors are required for mitotic progression and genome stability. NRDE2 (nuclear RNAi defective-2) is an evolutionarily conserved protein originally discovered for its role in nuclear RNA interference (RNAi) and heritable gene silencing in <i>Caenorhabditis elegans</i> (<i>C. elegans</i>). The function of the human <i>NRDE2</i> gene remains poorly understood. Here we show that human NRDE2 is an essential protein required for suppressing intron retention in a subset of pre-mRNAs containing short, GC-rich introns with relatively weak 5' and 3' splice sites. NRDE2 preferentially interacts with components of the U5 small nuclear ribonucleoprotein (snRNP), the exon junction complex, and the RNA exosome. Interestingly, <i>NRDE2-</i>depleted cells exhibit greatly increased levels of genomic instability and DNA damage, as well as defects in centrosome maturation and mitotic progression. We identify the essential centriolar satellite protein, CEP131, as a direct NRDE2-regulated target. NRDE2 specifically binds to and promotes the efficient splicing of <i>CEP131</i> pre-mRNA, and depleting <i>NRDE2</i> dramatically reduces CEP131 protein expression, contributing to impaired recruitment of critical centrosomal proteins (e.g., γ-tubulin and Aurora Kinase A) to the spindle poles during mitosis. Our work establishes a conserved role for human <i>NRDE2</i> in RNA splicing, characterizes the severe genomic instability phenotypes observed upon loss of <i>NRDE2</i>, and highlights the direct regulation of <i>CEP131</i> splicing as one of multiple mechanisms through which such phenotypes might be explained.