CX3C chemokine receptor 1
cell surface
TGF-β1 Downregulates the Expression of CX<sub>3</sub>CR1 by Inducing miR-27a-5p in Primary Human NK Cells.
Activity of human natural killer (NK) cells against cancer cells is deeply suppressed by TGF-β1, an immunomodulatory cytokine that is released and activated in the tumor microenvironment. Moreover, our previous data showed that TGF-β1 modifies the chemokine receptor repertoire of NK cells. In particular, it decreases the expression of CX<sub>3</sub>CR1 that drives these effectors toward peripheral tissues, including tumor sites. To identify possible mechanisms mediating chemokine receptors modulation, we analyzed the microRNA profile of TGF-β1-treated primary NK cells. The analysis pointed out miR-27a-5p as a possible modulator of CX<sub>3</sub>CR1. We demonstrated the functional interaction of miR-27a-5p with the 3' untranslated region (3'UTR) of CX<sub>3</sub>CR1 mRNA by two different experimental approaches: by the use of a luciferase assay based on a reporter construct containing the CX<sub>3</sub>CR1 3'UTR and by transfection of primary NK cells with a miR-27a-5p inhibitor. We also showed that the TGF-β1-mediated increase of miR-27a-5p expression is a consequence of miR-23a-27a-24-2 cluster induction. Moreover, we demonstrated that miR-27a-5p downregulates the surface expression of CX<sub>3</sub>CR1. Finally, we showed that neuroblastoma cells induced in resting NK cells a downregulation of the CX<sub>3</sub>CR1 expression that was paralleled by a significant increase of miR-27a-5p expression. Therefore, the present study highlights miR-27a-5p as a pivotal TGF-β1-induced regulator of CX<sub>3</sub>CR1 expression.