Gamma-aminobutyric acid receptor subunit beta-3
GABA-A receptor activity
Cryo-EM structure of the human α1β3γ2 GABA&lt;sub&gt;A&lt;/sub&gt; receptor in a lipid bilayer.
Type A γ-aminobutyric acid (GABA<sub>A</sub>) receptors are pentameric ligand-gated ion channels and the main drivers of fast inhibitory neurotransmission in the vertebrate nervous system<sup>1,2</sup>. Their dysfunction is implicated in a range of neurological disorders, including depression, epilepsy and schizophrenia<sup>3,4</sup>. Among the numerous assemblies that are theoretically possible, the most prevalent in the brain are the α1β2/3γ2 GABA<sub>A</sub> receptors<sup>5</sup>. The β3 subunit has an important role in maintaining inhibitory tone, and the expression of this subunit alone is sufficient to rescue inhibitory synaptic transmission in β1-β3 triple knockout neurons<sup>6</sup>. So far, efforts to generate accurate structural models for heteromeric GABA<sub>A</sub> receptors have been hampered by the use of engineered receptors and the presence of detergents<sup>7-9</sup>. Notably, some recent cryo-electron microscopy reconstructions have reported 'collapsed' conformations<sup>8,9</sup>; however, these disagree with the structure of the prototypical pentameric ligand-gated ion channel the Torpedo nicotinic acetylcholine receptor<sup>10,11</sup>, the large body of structural work on homologous homopentameric receptor variants<sup>12</sup> and the logic of an ion-channel architecture. Here we present a high-resolution cryo-electron microscopy structure of the full-length human α1β3γ2L-a major synaptic GABA<sub>A</sub> receptor isoform-that is functionally reconstituted in lipid nanodiscs. The receptor is bound to a positive allosteric modulator 'megabody' and is in a desensitized conformation. Each GABA<sub>A</sub> receptor pentamer contains two phosphatidylinositol-4,5-bisphosphate molecules, the head groups of which occupy positively charged pockets in the intracellular juxtamembrane regions of α1 subunits. Beyond this level, the intracellular M3-M4 loops are largely disordered, possibly because interacting post-synaptic proteins are not present. This structure illustrates the molecular principles of heteromeric GABA<sub>A</sub> receptor organization and provides a reference framework for future mechanistic investigations of GABAergic signalling and pharmacology.