Adhesion G protein-coupled receptor G3
adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway
Structures of the glucocorticoid-bound adhesion receptor GPR97-G<sub>o</sub> complex.
Adhesion G-protein-coupled receptors (GPCRs) are a major family of GPCRs, but limited knowledge of their ligand regulation or structure is available<sup>1-3</sup>. Here we report that glucocorticoid stress hormones activate adhesion G-protein-coupled receptor G3 (ADGRG3; also known as GPR97)<sup>4-6</sup>, a prototypical adhesion GPCR. The cryo-electron microscopy structures of GPR97-G<sub>o</sub> complexes bound to the anti-inflammatory drug beclomethasone or the steroid hormone cortisol revealed that glucocorticoids bind to a pocket within the transmembrane domain. The steroidal core of glucocorticoids is packed against the 'toggle switch' residue W<sup>6.53</sup>, which senses the binding of a ligand and induces activation of the receptor. Active GPR97 uses a quaternary core and HLY motif to fasten the seven-transmembrane bundle and to mediate G protein coupling. The cytoplasmic side of GPR97 has an open cavity, where all three intracellular loops interact with the G<sub>o</sub> protein, contributing to the high basal activity of GRP97. Palmitoylation at the cytosolic tail of the G<sub>o</sub> protein was found to be essential for efficient engagement with GPR97 but is not observed in other solved GPCR complex structures. Our work provides a structural basis for ligand binding to the seven-transmembrane domain of an adhesion GPCR and subsequent G protein coupling.