NAD(+) hydrolase SARM1
NAD+ nucleosidase activity
TIR domains of plant immune receptors are NAD<sup>+</sup>-cleaving enzymes that promote cell death.
Plant nucleotide-binding leucine-rich repeat (NLR) immune receptors activate cell death and confer disease resistance by unknown mechanisms. We demonstrate that plant Toll/interleukin-1 receptor (TIR) domains of NLRs are enzymes capable of degrading nicotinamide adenine dinucleotide in its oxidized form (NAD<sup>+</sup>). Both cell death induction and NAD<sup>+</sup> cleavage activity of plant TIR domains require known self-association interfaces and a putative catalytic glutamic acid that is conserved in both bacterial TIR NAD<sup>+</sup>-cleaving enzymes (NADases) and the mammalian SARM1 (sterile alpha and TIR motif containing 1) NADase. We identify a variant of cyclic adenosine diphosphate ribose as a biomarker of TIR enzymatic activity. TIR enzymatic activity is induced by pathogen recognition and functions upstream of the genes <i>enhanced disease susceptibility 1</i> (<i>EDS1</i>) and <i>N requirement gene 1</i> (<i>NRG1</i>), which encode regulators required for TIR immune function. Thus, plant TIR-NLR receptors require NADase function to transduce recognition of pathogens into a cell death response.