Putative divalent cation/proton antiporter TMEM165
calcium ion transport
The human Golgi protein TMEM165 transports calcium and manganese in yeast and bacterial cells.
Cases of congenital disorders of glycosylation (CDG) have been associated with specific mutations within the gene encoding the human Golgi TMEM165 (transmembrane protein 165), belonging to UPF0016 (uncharacterized protein family 0016), a family of secondary ion transporters. To date, members of this family have been reported to be involved in calcium, manganese, and pH homeostases. Although it has been suggested that TMEM165 has cation transport activity, direct evidence for its Ca<sup>2+</sup>- and Mn<sup>2+</sup>-transporting activities is still lacking. Here, we functionally characterized human TMEM165 by heterologously expressing it in budding yeast (<i>Saccharomyces cerevisiae</i>) and in the bacterium <i>Lactococcus lactis</i> Protein production in these two microbial hosts was enhanced by codon optimization and truncation of the putatively autoregulatory N terminus of TMEM165. We show that TMEM165 expression in a yeast strain devoid of Golgi Ca<sup>2+</sup> and Mn<sup>2+</sup> transporters abrogates Ca<sup>2+</sup>- and Mn<sup>2+</sup>-induced growth defects, excessive Mn<sup>2+</sup> accumulation in the cell, and glycosylation defects. Using bacterial cells loaded with the fluorescent Fura-2 probe, we further obtained direct biochemical evidence that TMEM165 mediates Ca<sup>2+</sup> and Mn<sup>2+</sup> influxes. We also used the yeast and bacterial systems to evaluate the impact of four disease-causing missense mutations identified in individuals with TMEM165-associated CDG. We found that a mutation leading to a E108G substitution within the conserved UPF0016 family motif significantly reduces TMEM165 activity. These results indicate that TMEM165 can transport Ca<sup>2+</sup> and Mn<sup>2+</sup>, which are both required for proper protein glycosylation in cells. Our work also provides tools to better understand the pathogenicity of CDG-associated <i>TMEM165</i> mutations.