



ORIGINAL ARTICLE Year : 2013 | Volume : 6 | Issue : 4 | Page : 267-272

Reproductive parameters and oxidative stress status of male rats fed with low and high salt diet



Bolanle O Iranloye, Gabriel O Oludare, Ayodele O Morakinyo, Naomi A Esume, Lucy C Ekeh

Department of Physiology, College of Medicine of the University of Lagos, Lagos, Nigeria





Date of Submission 12-Sep-2013 Date of Decision 06-Nov-2013 Date of Acceptance 27-Nov-2013 Date of Web Publication 3-Feb-2014

Correspondence Address:

Bolanle O Iranloye

Department of Physiology, College of Medicine of the University of Lagos, Surulere 23401, Lagos

Nigeria

Source of Support: None, Conflict of Interest: None Check 2

DOI: 10.4103/0974-1208.126308



Abstract

Background: Deficiency of minerals and micronutrients has been reported to impair the process of spermatogenesis. Historically, salt has been used by women on their husbands to increase their libido, however, the role of salt diet on sperm parameters are yet to be ascertained. AIM: The present study was designed to determine the effect of low and high salt diet on sperm parameters, oxidative status and reproductive hormone levels of male rats. Materials and Methods: A total of 18 rats were divided into three groups: Group I: (control) received 0.3% salt diet, Group II: low salt (received 0.14% salt diet) and Group III: high salt (received 8% salt diet). All animals were treated for 6 weeks; after which epididymal sperm parameters; oxidative stress markers (malondialdehyde, glutathione, catalase and superoxide dismutase) in the testes and epididymal tissues, as well as follicle stimulating hormone (FSH), luteinizing hormone (LH) and testosterone levels were determined. Results: The results showed decreased sperm count in the low salt diet rats while increased sperm count was observed in the high salt diet treated rats. Both low salt and high salt diet fed rats exhibited increased abnormal sperm cells and increased epididymal oxidative stress when compared with their respective control. FSH and testosterone levels were increased in the high salt fed rats while LH level was decreased when compared with the control values. Conclusion: This study suggests that both low and high salt diet play a negative role in the fertility of male rats.

Keywords: Oxidative stress, salt, sperm count, sperm morphology, testosterone

How to cite this article:

Iranloye BO, Oludare GO, Morakinyo AO, Esume NA, Ekeh LC. Reproductive parameters and oxidative stress status of male rats fed with low and high salt diet. J Hum Reprod Sci 2013;6:267-72

How to cite this URL:

Iranloye BO, Oludare GO, Morakinyo AO, Esume NA, Ekeh LC. Reproductive parameters and oxidative stress status of male rats fed with low and high salt diet. J Hum Reprod Sci [serial online] 2013 [cited 2019 Feb 26];6:267-72. Available from: http://www.jhrsonline.org/text.asp?2013/6/4/267/126308

Introduction

Materials and Methods

Group I: 0.3% NaCl (control normal diet [27] )

) Group II: 0.14% NaCl (low salt diet)

Group III: 8% NaCl (high salt diet).

2

2

Results

Table 1: Sperm characteristics of male rats in control and other experimental rats



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Table 2: LH, FSH and testosterone levels of male rats in control and other experimental rats



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Figure 1: Effect of low salt and high salt diet on malondialdehyde level in the testes and epididymis of rats. Values represent mean ± standard error of mean (n=6), P<0.05 compared with control group



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Figure 2: Effect of low salt and high salt diet on superoxide dismutase activity in the testes and epididymis of rats. Values represent mean ± standard error of mean (n=6), *P<0.05 compared with control group



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Figure 3: Effect of low salt and high salt diet on glutathione activity in the testes and epididymis of rats. Values represent mean ± standard error of mean (n=6), *P<0.05 compared with control group



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Figure 4: Effect of low salt and high salt diet on catalase activity in the testes and epididymis of rats. Values represent mean ± standard error of mean (n=6), *P<0.05 compared with control group



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Discussion

Sertoli cells

More Details

Conclusion

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