JERUSALEM — Three top Israeli ministers on Tuesday denied a report that their intelligence services had spied on the closed-door negotiations over Iran’s nuclear program, as tensions continued to mount between Washington and Jerusalem.

“There is no such thing as Israel spying on the Americans,” the defense minister, Moshe Yaalon, said at a pre-Passover toast, according to a transcript provided by his office. Mr. Yaalon said he had checked and found no complaint from the United States to Israeli intelligence services about such spying. “There is a strict prohibition on that,” he said.

Yuval Steinitz, the minister for strategic affairs, who is in Europe lobbying officials about the Iran talks, said on Israeli television that “these claims are baseless and we reject them outright.” Avigdor Lieberman, Israel’s foreign minister, called the report in The Wall Street Journal on Tuesday “incorrect and inaccurate,” but hinted that Israel may have gleaned information about the talks from spying on the Iranian side.

“Clearly, the State of Israel has various security interests, and clearly we have good intelligence services,” Mr. Lieberman said on Army Radio. “We do not spy on the United States. There are enough elements involved, such as Iranian elements, first and foremost.”