A MELBOURNE real estate firm that claimed to have raised $76,070 from a charity golf day, but donated only a quarter to the Murdoch Children's Research Institute could face legal action from the research centre.

Institute director Terry Dwyer said Armadale agent Abercromby's had sent two cheques for about $20,000 and $74, despite claims on the company's website that the entire proceeds had been handed over.

Real estate agent Tim Derham and golfers enjoying the charity day.

Professor Dwyer said he had sent a letter to Abercromby's demanding an explanation over the significant discrepancy.

"We do see this in the context of people trying to assist us, but we think in this case, the outcome was not what we'd expect or think was appropriate, which is why we've taken it up with the organisation," Professor Dwyer said.